Double Staining of Tobacco-mosaic-virus-infected Material Using Phosphotungstic Acid and Uranyl Acetate During Dehydration
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Hot Alcoholic Phosphotungstic Acid and Uranyl Acetate as Routine Stains for Thick and Thin Sections
One of the potential advantages of high-voltage electron microscopes is that their greater penetrating power allows the use of thick sections (0.22 lt) to study three-dimensional cytoarchitecture . So far, this advantage has been limited because conventional methods of staining only allow the penetration of heavy metal solutions into sections to a depth of a few hundred Angstroms . For example,...
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After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...
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The proteins synthesized in tissue infected with tobacco mosaic virus.
News and Notes Meeting of the American Chemical Society: Walter J. Murphy 541 The Effects of Allithiamine on Some Thiamine-Requiring Organisms: V. G. Lilly, H. L. Barnett, and B. G. Anderson ..........548 The Effect of Cortisone upon the Therapeutic Efficacy of Antibiotics: Ernest .Tawetz and Elizabeth R. Merrill...................549 Resumption of Heartb'eat in the Rabbit Embryo after Exposure...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1968
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-3-2-309